A title, introduction, methods, results (with figures and/or tables), discussion, conclusion
include 5 refrences articles and in text citing. (not the articles attached
What are your aims?: To investigate the evolutionary response of Pseudomonasd fluorescens bacteria to ecological opportunity.
Homogenous and heterogenous environment. Homogenous meaning it’s all the same. Equal contact to oxygen.
What are your hypotheses?: From genetically identical stocks, mutant forms will evolve to exploit new environmental conditions. (Over 4 weeks)
1 What are the (pre) treatments that were applied to the bacteria?
Pre Treatment 1: Shaken
Pre Treatment 2: Stationary
1. How were the treatments applied?
All cultures were kept at room temperature in kings medium broth in 5ml flasks with lids attached loosely to allow for oxygen flow
Treatment 1: Shaken – Cultures were continuously shaken on an orbital shaker at 250 RPM for 10 days.
Treatment 2: Stationary – Cultures were kept stationary on bench top for 10 days
2. What variables are measured?
Number and proportion of colonies belonging to either the “Smooth” morphotype (SM). “Wrinkly Spreader” morphotype (WS). Or
“Fuzzy Spreader” morphotype (FS). When diluted and plated on agar.
3. How are they to be measured? Using what equipment?
After (pre) treatments were applied a 50uL sample of bacterial culture was diluted to 2.5×10-7 and spread on a kings medium agar plate using a modified version of standard sterile technique. Colonies were allowed to grow on agar plates at room temperature for 2 days before being stored in the refrigerator at 4°C for 5 days. Plates were then stored for number and proportion of colonies belonging to each morphotype by visual analysis of colony shape.
4. How was it ensured that the variables were measured without bias? When plating treatment colonies cultures, Culture identity was kept blinded. Some individuals from student groups counted the plate colonies without knowing the culture identity.
5. How will the data be collated and what is the level of replication? Increasing sample size to decreases error. The number and proportion of colonies of each morphotype for each treatment will be collated across all classes (excel attached and outlines the different morphology data)