Frog Lab Report-Biology

Part one:

Data

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Stimulus (mV) CAP (mV)

10                    0.01

20                    0.01

30                    -0.02

40                    -0.01

50                    -0.02

60                    0.06

70                    0.28

80                    1.12

90                    2.25

100                  3.16

110                  3.77

120                  4.18

130                  4.41

140                  4.55

150                  4.64

160                  4.72

170                  4.79

180                  4.82

190                  4.91

200                  4.88

 

 

 

Questions

 

  1. Please give the graph a name ^ what it would be?

 

 

 

 

  1. How does a CAP differ from a single action potential?

 

  1. Action potentials are said to be all-or-none responses. Why does the frog sciatic nerve give a graded response?

 

  1. In this activity, you examined the effect of increasing stimulus intensity on the nerve. What’s another stimulus parameter that might also affect the nerve’s tendency to generate a CAP?

 

  1. What is the Min. Stimulus for a Max. CAP (mV)?

 

 

Part 2:

 

 

please label in the graph ^

 

 

 

 

 

Part3

Data

 

 

Interpulse Interval (ms) Frequency
(Hz)
Interpulse Interval (ms) Frequency
(Hz)
4 250 1.6 625
3.5 285.7 1.5 666.7
3 333.3 1.4 714.3
2.5 400 1.3 769.2
2 500 1.2 833.3
1.9 526.3 1.1 909.1
1.8 555.6 1 1000
1.7 588.2

 

Questions

  1. What is the Stimulus for a Max. CAP (mV)?

 

 

 

Part 4

Data

 

Interpulse Interval (ms)           Amplitude 2nd CAP (mV)

4.0                                           4.83

3.5                                           -2.45

3.0                                           -38.00

2.5                                           -0.03

2.0                                           -0.05

1.9                                           0.00

1.8                                           -0.08

1.7                                           -0.08

1.6                                           -0.05

1.5                                           -0.04

1.4                                           -0.08

1.3                                           -0.01

1.2                                           -0.02

1.1                                           -0.03

1.0                                           -0.03

 

Questions

  1. Please make sure to label the graph below.

 

 

In the table below, enter the interpulse interval at which you detect a decrease in the magnitude of the second CAP. This marks the end of the relative refractory period.

Enter the interpulse interval at which the second CAP first disappears. This marks the end of the absolute refractory period.

Interpulse Interval (ms)
End of relative refractory period
End of absolute refractory period
   

 

 

 

  1. Explain the difference between the relative and absolute refractory periods?

 

  1. Briefly describe the cellular events responsible for the refractory period. (Hint: Think about the mechanism of repolarization)?

 

 

 

Part 5

Data

 

Variable Value
Distance between recording electrodes (mm) 30
Time interval between CAP Proximal and CAP Distal (ms) 0.54
Conduction velocity (m/s) 55.56

 

Questions

  1. Based on your calculation for CAP conduction velocity, how long would it take the CAP to travel the length of the sciatic nerve? Assume a total length of 100 mm.

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